105. MACROMONAS MEDIUM 2

(NH₄)₂SO₄ 0.5 g

MgSO₄ × 7 H₂O 0.2 g

Casein acidic hydrolysate 1.0 g

Na-acetate × 3 H₂O 1.0 g, or succinate, or benzoate 0.5 g

Na₂S₂O₃ × 5 H₂O 0.5 g

Catalase 2.0 mg

Vitamin solution (see below) 1.0 ml

Distilled water 1000.0 ml

Vitamin solution:

Biotin 200.0 mg

Folic acid 20.0 mg

Pyridoxine-HCl 100.0 mg

Thiamine-HCl 50.0 mg

Riboflavin 100.0 mg

Nicotinic acid 50.0 mg

DL-Pantothenic acid 50.0 mg

Vitamin B₁₂ 1.0 mg

p-Aminobenzoic acid 50.0 mg

Distilled water 1000.0 ml

pH 7.2-7.4

Autoclave at 121°C for 15 min. Sterilize catalase and vitamins separately from the main medium by filtration. Thiosulfate should also better be autoclaved separately at 121°C for 15 min.