109. DESULFOVIBRIO MEDIUM

Solution 1:

K2HPO4 0.5 g

NH4Cl 1.0 g

Na2SO4 1.0 g

CaCl2 2 H2O 0.1 g

MgSO4 7 H2O 2.0 g

Na DL-lactate 2.0 g

Yeast extract 1.0 g

Resazurin 1.0 g

Distilled water 980.0 ml

Solution 2:

FeSO4 7 H2O 0.5 g

Distilled water 10.0 ml

Solution 3:

Na-thioglycollate 0.1 g

Ascorbic acid 0.1 g

Distilled water 10.0 ml

Solution 4:

Trace element solution (see below) 1.0 ml

Trace element solution:

FeCl2 4 H2O 1.5 g

ZnCl2 68.0 mg

MnCl2 4 H2O 100.0 ml

H3BO3 62.0 mg

CoCl2 6 H2O 120.0 ml

Na2MoO4 2 H2O 24.0 mg

HCl, 0.05 M 1000.0 ml

pH 7.4-7.8

Autoclave at 121C for 15 min under an atmosphere of N2. Solution 1 is boiled before sterilization, being blown with N2.

 

Updated 21.06.2021