128. HIRSCH MEDIUM

KH2PO4 1.36 g

Na2HPO4 2.15 g

MgSO4 7 H2O 0.2 g

MnSO4 5 H2O 1.05 g

(NH4)2SO4 0.5 g

CaCl2 2 H2O 5.97 mg

Na2MoO4 2 H2O 1.5 mg

Methanol 5.0 ml

Distilled water to 1000.0 ml

pH 7.0

Autoclave at 121C for 15 min. Sterilize methanol by filtration and add to the medium after autoclaving.

 

Updated 09.08.2021