13. POTATO-DEXTROSE AGAR (PDA)

Grated potato 200.0 g

Dextrose 20.0 g

Agar 20.0 g

Tap water 1000.0 ml

Boil 200 g scrubbed and sliced potatoes in 1000.0 ml water for 1 hour, filter cold through a cotton-gauze filter, add water to the initial volume, add glucose and agar. Do not use new potatoes.

pH 6.5-7.0

Autoclave at 121C 15 min.

Updated 25/05/2020