133. COLBY AND ZATMAN MEDIUM WITH METHANOL

K2HPO4 1.2 g

KH2PO4 0.62 g

CaCl2 6 H2O 0.05 g

MgSO4 7 H2O 0.2 g

NaCl 0.1 g

FeCl3 6 H2O 1.0 mg

(NH4)2SO4 0.5 g

CuSO4 5 H2O 5.0 g

MnSO4 5 H2O 10.0 g

Na2MoO4 2 H2O 10.0g

H3BO3 10.0 g

ZnSO4 7 H2O 70.0 g

CoCl2 6 H2O 5.0g

Purified agar 15.0 g

Methanol 1.0 ml

Distilled water 1000.0 ml

pH 6.8

Autoclave at 121C for 15 min. Methanol is filter sterilized.

 

Updated 09.08.2021