134. METHYLOTROPH MEDIUM 1

KH2PO4 2.0 g

(NH4)2SO4 2.0 g

MgSO4 7 H2O 0.025 g

NaCl 0.5 g

FeSO4 7 H2O 0.0 2 g

Methanol 5.0 ml

Distilled water 1000.0 ml

pH 7.0

Autoclave at 121C for 15 min. Methanol is filter sterilized. Methanol may be replacement 30 ml 10 % solution of methylamine, autoclave at 111C for 30 min.

 

Updated 28/05/2020