134. METHYLOTROPH MEDIUM 1

KH2PO4 2.0 g

(NH4)2SO4 2.0 g

MgSO4 × 7 H2O 0.025 g

NaCl 0.5 g

FeSO4 × 7 H2O 0.02 g

Methanol 5.0 ml

Distilled water 1000.0 ml

pH 7.0

Autoclave at 121°C for 15 min. Methanol is filter sterilized. Methanol may be replacement 30 ml 10 % solution of methylamine, autoclave at 111°C for 30 min.

 

Updated 01.02.2024