135. METHYLOTROPH MEDIUM 2

KH₂PO₄ 0.8 g

Na₂HPO₄ × 12 H₂O 3.0 g

(NH₄)₂SO₄ 0.8 g

MgSO₄ × 7 H₂O 0.1 g

FeSO₄ × 7 H₂O 10.0 mg

Methanol 5.0 ml

Trace element solution (see below) 1.0 ml

Distilled water 1000.0 ml

Trace element solution:

CaCl₂ × 2 H₂O 1.25 g

CoCl₂ × 6 H₂O 1.25 g

MnSO₄ × 4 H₂O 0.1 g

ZnSO₄ × 7 H₂O 1.25 g

Na₂MoO₄ × 2 H₂O 50.0 mg

CuCl₂ × 2 H₂O 250.0 mg

Distilled water 250.0 ml

pH 7.0-7.2

Autoclave at 121°C for 15 min. Methanol is filter sterilized. Methanol may be replacement 30 ml 10 % solution of methylamine, autoclave at 111°C for 30 min.

 

Updated 01.02.2024