139. POSTGATE MEDIUM B FOR SULFATE REDUCERS

Solution 1:

NaCl 1.0 g

KH₂PO₄ 0.5 g

NH₄Cl 1.0 g

CaSO₄ × 2 H₂O 1.0 g

MgSO₄ × 7 H₂O 2.0 g

Na-lactate 3.5 g

Yeast extract 1.0 g

Tap water 980.0 ml

Dissolve ingredients and sparge medium with O₂-free N₂ gas for 10-15 minutes

Solution 2:

Ascorbic acid 1.0 g

Thioglycolic acid 1.0 g

Distilled water 10.0 ml

Solution 3:

FeSO₄ × 7 H₂O 0.5 g

Distilled water 10.0 ml

pH 7.4

Autoclave solution 1 at 121°C for 15 min, solutions 2 and 3 separately autoclave at 111°C for 30 min.

 

Updated 27.05.2023