152. TRYPTONE THIOGLYCOLATE MEDIUM

Solution 1:

K₂HPO₄ 5.45 g

KH₂PO₄ 1.2 g

MgSO₄ × 7 H₂O 25.0 mg

CaCl₂ × 2 H₂O 15.0 mg

FeSO₄ × 7 H₂O 10.0 mg

MnCl₂ × 4 H₂O 2.0 mg

CoCl₂ × 6 H₂O 2.5 mg

Na₂MoO₄ × 2 H₂O 2.5 mg

Peptone 2.0 g

Tryptone 2.0 g

Yeast extract 6.0 g

Na-thioglycolate 0.5 g

Distilled water 950.0 ml

Solution 2:

Glucose 20.0 g

Distilled water 50.0 ml

pH 7.5

Autoclave solution 1 at 121°C for 15 min, solution 2 at 111°C for 30 min.

 

Updated 27.05.2023