152. TRYPTONE THIOGLYCOLATE MEDIUM

Solution 1:

K2HPO4 5.45 g

KH2PO4 1.2 g

MgSO4 7 H2O 25.0 mg

CaCl2 2 H2O 15.0 mg

FeSO4 7 H2O 10.0 mg

MnCl2 4 H2O 2.0 mg

CoCl2 6 H2O 2.5 mg

Na2MoO4 2 H2O 2.5 mg

Peptone 2.0 g

Tryptone 2.0 g

Yeast extract 6.0 g

Na-thioglycolate 0.5 g

Distilled water 950.0 ml

Solution 2:

Glucose 20.0 g

Distilled water 50.0 ml

pH 7.5

Autoclave solution 1 at 121C for 15 min, solution 2 at 111C for 30 min.

 

Updated 10.08.2021