161. RHIZOBIUM MEDIUM

Yeast extract 1.0 g

Mannitol 10.0 g

Agar 15.0 g

Soil extract (see below) 200.0 ml

Distilled water 800.0 ml

Soil extract:

Air-dried garden soil 80.0 g

Na₂CO₃ 0.2 g

Distilled water 200.0 ml

Autoclave at 121 C for 15 min.

pH 7.2

Sterilize at 121 C for 15 min.