180. DESULFOVIBRIO CARBINOLICUS MEDIUM

Solution 1:

Na2SO4 3.0 g

KH2PO4 0.2 g

NH4Cl 0.3 g

NaCl 1.0 g

MgCl2 6 H2O 0.4 g

KCl 0.5 g

CaCl2 2 H2O 0.15 g

Distilled water 870.0 ml

Solution 2:

Trace element solution SL-10 (see below) 1.0 ml

Solution 3:

NaHCO3 5.0 g

Distilled water 100.0 ml

Solution 4:

Ethanol 0.7 g

Casamino acids 0.1 g

Yeast extract 0.1 g

Distilled water 10.0 ml

Solution 5:

Vitamin solution (see below) 10.0 ml

Solution 6:

Na2S 9 H2O 0.4 g

Distilled water 10.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl2 4 H2O 1.5 g

ZnCl2 70.0 mg

MnCl2 4 H2O 100.0 mg

H3BO3 6.0 mg

CoCl2 6 H2O 190.0 mg

CuCl2 2 H2O 2.0 mg

NiCl2 6 H2O 24.0 mg

Na2MoO4 2 H2O 36.0 mg

Distilled water 990.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine-HCl 10.0 mg

Thiamine-HCl 5.0 mg

Riboflavin 5.0 mg

Nicotinic acid 5.0 mg

Ca DL-pantothenate 5.0 mg

Vitamin B12 0.1 mg

p-Aminobenzoic acid 5.0 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

Solution 1 is boiled before sterilization for a few minutes being flushed with gas mixture of 80% N2 and 20% CO2 with sodium bicarbonate added until an equilibrium pH of 6.9-7.1. Solution 1 is autoclaved under this gas mixture at 121C for 15 min. Solution 2 is autoclaved under 100% N2 at 121C for 15 min. Vitamin solution is filter sterilized. Others solutions autoclave at 121C for 15 min and add to base medium aseptically.

Final pH of the complete medium 7.1-7.4

 

Updated 21.06.2021