188. Natronobacterium MEDIUM

Solution 1:

KH2PO4 1.0 g

KCl 1.0 g

NH4Cl 1.0 g

MgSO4 × 7 H2O 0.24 g

CaSO4 × 2 H2O 0.17 g

Trace element solution SL-10 (see below) 1.0 ml

NaCl 200.0 g

Glutamate 1.0 g

Yeast extract 5.0 g

Casamino acids 5.0 g

Agar, if necessary (heat and dissolve it before adding sodium chloride) 20.0 g

Distilled water to 1000.0 ml

pH 6.5

Solution 2:

Na2CO3 5.0 g

Distilled water 50.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl2 × 4 H2O 1.5 g

ZnCl2 70.0 mg

MnCl2 × 4 H2O 100.0 mg

H3BO3 6.0 mg

CoCl2 × 6 H2O 190.0 mg

CuCl2 × 2 H2O 2.0 mg

NiCl2 × 6 H2O 24.0 mg

Na2MoO4 × 2 H2O 36.0 mg

Distilled water 990.0 ml

pH 9.0-9.5

Autoclave separately at 121°C for 15 min and mix aseptically.

 

Updated 1.02.2024