214. STARCH AMMONIA AGAR

Starch (soluble) 10.0 g

K₂HPO₄ 1.0 g

MgSO₄ × 7 H₂O 1.0 g

NaCl 1.0 g

(NH₄)₂SO₄ 2.0 g

CaCO₃ 2.0 g

Salt solution A (see below) 1.0 ml

Agar 20.0 g

Distilled water 1000.0 ml

Salt solution A:

FeSO₄ × 7 H₂O 0.1 g

MnCl₂ × 4 H₂O 0.1 g

ZnSO₄ × 7 H₂O 0.1 g

Distilled water 100.0 ml

pH 7.0-7.4

Autoclave separately at 121°C 15 min.

 

Updated 1.02.2024