221. MEDIUM FOR METHANOTROPHIC BACTERIA

KNO3 1.0 g

KH2PO4 0.7 g

MgSO4 7 H2O 0.2 g

CaCl2 2 H2O 0.02 g

Na2HPO4 12 H2O 1.5 g

EDTA 5.0 mg

FeSO4 7 H2O 2.0 mg

ZnSO4 7 H2O 0.1 mg

MnCl2 4 H2O 0.03 mg

CoCl2 6 H2O 0.2 mg

CuCl2 2 H2O 0.1 mg

NiCl2 6 H2O 0.02 mg

Na2MoO4 2 H2O 0.03 mg

Distilled water 1000.0 ml

pH 6.7-7.1

Autoclave at 121C 15 min.

Cultivation under mixture of methane and air (1:1).

 

Updated 29/05/2020