222. MEDIUM FOR MARINE METHYLOTROPHIC BACTERIA

KH₂PO₄ 2.0 g

(NH₄)₂SO₄ 2.0 g

NaCl 30.0 g

MgSO₄ × 7 H₂O 0.025 g

FeSO₄ × 7 H₂O 0.0 2 g

Yeast extract 0.1 g

Methanol 5.0 ml

Biotin 0.01 mg

Distilled water 1000.0 ml

pH 7.0

Autoclave at 121°C 15 min. After autoclaving add filter sterilized methanol and biotin.

 

Updated 1.02.2024