222. MEDIUM FOR MARINE METHYLOTROPHIC BACTERIA

KH2PO4 2.0 g

(NH4)2SO4 2.0 g

NaCl 30.0 g

MgSO4 7 H2O 0.025 g

FeSO4 7 H2O 0.0 2 g

Yeast extract 0.1 g

Methanol 5.0 ml

Biotin 0.01 mg

Distilled water 1000.0 ml

pH 7.0

Autoclave at 121C 15 min. After autoclaving add filter sterilize methanol and biotin.

 

Updated 29/05/2020