224. MEDIUM FOR DESULFOTOMACULUM ALKALIPHILUM

Solution 1 (basic solution):

Na₂CO₃ 0.5 g

Na₂SO₄ 5.0 g

NaCl 5.0 g

Na formate 5.0 g

Yeast extract 1.0 g

Solution 2 (see below) 10.0 ml

Solution 3 (see below) 2.0 ml

Solution 4 (see below) 1.0 ml

Rezazurine traces

Distilled water 1000.0 ml

NaHCO₃ final concentration 8.0 g /1000.0ml

Na₂S × 9 H₂O final concentration 0.5 g/1000.0 ml

Solution 2:

KH₂PO₄ 0.2 g

MgCl₂ × 6 H₂O 0.1 g

NH₄Cl 1.0 g

KCl 0.2 g

Distilled water 1000.0 ml

Solution 3 (vitamin solution):

Biotin 10.0 mg

Folic acid 10.0 mg

Pyridoxine-HCl 50.0 mg

Riboflavine 25.0 mg

Thiamine-HBr 25.0 mg

Nicotine amide 25.0 mg

D-pantothenate 25.0 mg

Vitamin B₁₂ 0.5 mg

p-Aminobenzoic acid 25.0 mg

Thioctic acid 25.0 mg

Distilled water 500.0 ml

Store in refrigerator at +5°C.

Solution 4 (trace elements):

Na₂MoO₄ × 2 H₂O 20.0 mg

Fe(NH₄)₂(SO₄)₂ × 6 H₂O 400.0 mg

FeSO₄ × 7 H₂O 200.0 mg

ZnSO₄ × 7 H₂O 200.0 mg

MnCl₂ × 4 H₂O 720.0 mg

NiCl₂ × 6 H₂O 100.0 mg

CuSO₄ × 5 H₂O 20.0 mg

AlK(SO₄)₂ × 12 H₂O 20.0 mg

CoCl₂ × 6 H₂O 200.0 mg

H₃BO₃ 20.0 mg

HCl 5.0 ml

Distilled water 200.0 ml

pH of the autoclaved medium is 8.7-9.0

Vitamin solution is filter sterilized. Others solutions autoclave separately at 121°C for 15 min and add to the Solution 1 after cooling.

 

Updated 1.02.2024