224. MEDIUM FOR DESULFOTOMACULUM ALKALIPHILUM

Solution 1 (basic solution):

Na2CO3 0.5 g

Na2SO4 5.0 g

NaCl 5.0 g

Na formate 5.0 g

Yeast extract 1.0 g

Solution 2 (see below) 10.0 ml

Solution 3 (see below) 2.0 ml

Solution 4 (see below) 1.0 ml

Rezazurine traces

Distilled water 1000.0 ml

NaHCO3 final concentration 8.0 g /1000.0ml

Na2S 9 H2O final concentration 0.5 g/1000.0 ml

Solution 2:

KH2PO4 0.2 g

MgCl2 6 H2O 0.1 g

NH4Cl 1.0 g

KCl 0.2 g

Distilled water 1000.0 ml

Solution 3 (vitamin solution):

Biotin 10.0 mg

Folic acid 10.0 mg

Pyridoxine-HCl 50.0 mg

Riboflavine 25.0 mg

Thiamine-HBr 25.0 mg

Nicotine amide 25.0 mg

D-pantothenate 25.0 mg

Vitamin B12 0.5 mg

p-Aminobenzoic acid 25.0 mg

Thioctic acid 25.0 mg

Distilled water 500.0 ml

Store in refrigerator at +5C.

Solution 4 (trace elements):

Na2MoO4 2 H2O 20.0 mg

Fe(NH4)2(SO4)2 6 H2O 400.0 mg

FeSO4 7 H2O 200.0 mg

ZnSO4 7 H2O 200.0 mg

MnCl2 4 H2O 720.0 mg

NiCl2 6 H2O 100.0 mg

CuSO4 5 H2O 20.0 mg

AlK(SO4)2 12 H2O 20.0 mg

CoCl2 6 H2O 200.0 mg

H3BO3 20.0 mg

HCl 5.0 ml

Distilled water 200.0 ml

pH of the autoclaved medium is 8.7-9.0

Vitamin solution is filter sterilized. Others solutions autoclave separately at 121C for 15 min and add to the Solution 1 after cooling.

 

Updated 23.06.2021