229. Alkalibacter medium

(NH₄)₂SO₄ 1.0 g

NH₄Cl 0.4 g

Na₂S₂O₃ × 5 H₂O 0.1 g

K₂HPO₄ 0.5 g

MgSO₄ × 7 H₂O 0.1 g

CaCl₂ × 2 H₂O 0.05 g

NaCl 10.0 g

FeSO₄ × 7 H₂O 2.0 mg

Trace element solution (see below) 10.0 ml

Resazurin 0.01 g

Yeast extract 0.25 g

Tryptone 2.0 g

Glucose 5.0 g

Vitamin solution (see below) 10.0 ml

L-cysteine 0.5 g

Na₂CO₃ (5% w/v) 50.0 ml

Distilled water 930.0 ml

Trace element solution:

Nitrilotriacetic acid 12.8 g

FeSO₄ × 7 H₂O 0.1 g

MnCl₂ × 4 H₂O 0.1 g

CoCl₂ × 2 H₂O 0.17 g

CaCl₂ × 2 H₂O 0.1 g

ZnCl₂ 0.1 g

CuCl₂ × 2 H₂O 0.02 g

H₃BO₃ 0.01 g

Na₂MoO₄ × 2 H₂O 0.01 g

NaCl 1.0 g

Na₂SeO₄ 0.017 g

Distilled water 1000.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine  10.0 mg

Riboflavin 5.0 mg

Pantotenoic acid 5.0 mg

p-Aminobenzoic acid 5.0 mg

Thiamine-HCl 5.0 mg

Nicotinic acid 5.0 mg

Vitamin B₁₂ 0.1 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

Basal medium autoclave at 111°C for 30 min. Vitamin solution is filter sterilized. Others solutions autoclave at 121°C for 15 min (Na₂CO₃ is autoclaved under 100% N₂).

Final pH 9.0

 

Updated 1.02.2024