230. Caldithrix abyssi medium

Sea salt 37.9 g

NH₄Cl 0.33 g

KH₂PO₄ 0.33 g

Resazurin 0.5 mg

Yeast extract (20% w/v) 15.0 ml

Trace element solution (see below) 10.0 ml

Vitamin solution (see below) 10.0 ml

Na₂S × 9 H₂O (3% w/v) 20.0 ml

Selenite-tungstate solution (see below) 1.0 ml

Distilled water 900.0 ml

Trace element solution:

Nitrilotriacetic acid 12.8 g

FeSO₄ × 7 H₂O 0.1 g

MnCl₂ × 6 H₂O 0.1 g

CoCl₂ × 2 H₂O 0.17 g

CaCl₂ × 2 H₂O 0.1 g

ZnCl₂ 0.1 g

CuCl₂ × 2 H₂O 0.02 g

H₃BO₃ 0.01 g

Na₂MoO₄ × 2 H₂O 0.01 g

NaCl 1.0 g

Na₂SeO₄ 0.017 g

Distilled water 1000.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine-HCl 10.0 mg

Thiamine-HCl × 2 H₂O 5.0 mg

Riboflavin 5.0 mg

Nicotinic acid 5.0 mg

D-Ca-pantothenate 5.0 mg

Vitamin B₁₂ 0.1 mg

p-Aminobenzoic acid 5.0 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

Selenite-tungstate solution:

NaOH 0.5 g

Na₂SeO₃ × 5 H₂O 3 mg

Na₂WO₄ × 2 H₂O 4 mg

Distilled water 1000.0 ml

Prepare medium anaerobically under 80% N₂ + 20% CO₂ gas mixture. After autoclaving add from separately prepared, sterile anaerobic stock solutions NaHCO₃ (5% w/v) 50.0 ml. Vitamin solution is filter sterilized. Others solutions autoclave separately at 121°C for 15 min.

Final pH of the medium to 6.8

 

Updated 1/02/2024