230. Caldithrix abyssi medium

Sea salt 37.9 g

NH4Cl 0.33 g

KH2PO4 0.33 g

Resazurin 0.5 mg

Yeast extract (20% w/v) 15.0 ml

Trace element solution (see below) 10.0 ml

Vitamin solution (see below) 10.0 ml

Na2S 9 H2O (3% w/v) 20.0 ml

Selenite-tungstate solution (see below) 1.0 ml

Distilled water 900.0 ml

Trace element solution:

Nitrilotriacetic acid 12.8 mg

FeSO4 7 H2O 0.1 mg

MnCl2 6 H2O 0.1 mg

CoCl2 2 H2O 0.17 mg

CaCl2 2 H2O 0.1 mg

ZnCl2 0.1 mg

CuCl2 2 H2O 0.02 mg

H3BO3 0.01 mg

Na2MoO4 2 H2O 0.01 mg

NaCl 1.0 mg

Na2SeO4 0.017 mg

Distilled water 1000.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine-HCl 10.0 mg

Thiamine-HCl 2 H2O 5.0 mg

Riboflavin 5.0 mg

Nicotinic acid 5.0 mg

D-Ca-pantothenate 5.0 mg

Vitamin B12 0.1 mg

p-Aminobenzoic acid 5.0 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

Selenite-tungstate solution:

NaOH 0.5 g

Na2SeO3 5 H2O 3 mg

Na2WO4 2 H2O 4 mg

Distilled water 1000.0 ml

Prepare medium anaerobically under 80% N2 + 20% CO2 gas mixture. After autoclaving add from separately prepared, sterile anaerobic stock solutions NaHCO3 (5% w/v) 50.0 ml. Vitamin solution is filter sterilized. Others solutions autoclave separately at 121C for 15 min.

Final pH of the medium to 6.8

 

Updated 30/05/2020