233. Clostridium alkalicellulosi medium

NH₄Cl 0.5 g

KH₂PO₄ 0.2 g

MgCl₂ × 6 H₂O 0.1 g

KCl 0.2 g

Trace element solution SL-10 (see below) 1.0 ml

Selenite-tungstate solution (see below) 1.0 ml

Na₂CO₃ 1.0 g

NaHCO₃ 7.6 g

NaCl 10.0 g

Yeast extract 0.2 g

Cellobiose 3.0 g

Na₂S × 9 H₂O 0.5 g

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl₂ × 4 H₂O 1.5 g

ZnCl₂ 70.0 mg

MnCl₂ × 4 H₂O 100.0 mg

H₃BO₃ 6.0 mg

CoCl₂ × 6 H₂O 190.0 mg

CuCl₂ × 2 H₂O 2.0 mg

NiCl₂ × 6 H₂O 24.0 mg

Na₂MoO₄ × 2 H₂O 36.0 mg

Distilled water 990.0 ml

Selenite-tungstate solution:

NaOH 0.5 g

Na₂SeO₃ × 5 H₂O 3 mg

Na₂WO₄ × 2 H₂O 4 mg

Distilled water 1000.0 ml

Dissolve ingredients except cellobiose and sulfide, flush medium with N₂ gas for 30–60 min, dispense under N₂ gas atmosphere and autoclave at 121°C 15 min. Add cellobiose after autoclaving from an anoxic stock solution sterilized by filtration and sulfide from a sterile (autoclave at 121°C 15 min), anoxic stock solution prepared under N₂.

Final pH of the medium 8.8-9.0.

 

Updated 27.05.2023