233. Clostridium alkalicellulosi medium

NH4Cl 0.5 g

KH2PO4 0.2 g

MgCl2x 6 H2O 0.1 g

KCl 0.2 g

Trace element solution SL-10 (see below) 1.0 ml

Selenite-tungstate solution (see below) 1.00 ml

Na2CO31.0 g

NaHCO37.6 g

NaCl 10.0 g

Yeast extract 0.2 g

Cellobiose 3.0 g

Na2Sx 9 H2O 0.5 g

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl3 x 4 H2O 1.5 g

ZnCl2 70.0 mg

MnCl2 x 4 H2O 100.0 mg

H3BO3 6.0 mg

CoCl2 x 6 H2O 190.0 mg

CuCl2 x 2 H2O 2.0 mg

NiCl2 x 6 H2O 24.0 mg

Na2MoO4 x 2 H2O 36.0 mg

Distilled water 990.0 ml

Selenite-tungstate solution:

NaOH 0.5 g

Na2SeO3 x 5 H2O 3 mg

Na2WO4 x 2 H2O 4 mg

Distilled water 1000.0 ml

Dissolve ingredients except cellobiose and sulfide, flush medium with N2 gas for 3060 min., dispense under N2 gas atmosphere and autoclave at 121 C 15 min. Add cellobiose after autoclaving from an anoxic stock solution sterilized by filtration and sulfide from a sterile (sterilize at 121 C 15 min.), anoxic stock solution prepared under N2.

Final pH of the complete medium 8.8-9.0

Updated 02/06/2010