233. Clostridium alkalicellulosi medium

NH4Cl 0.5 g

KH2PO4 0.2 g

MgCl2 6 H2O 0.1 g

KCl 0.2 g

Trace element solution SL-10 (see below) 1.0 ml

Selenite-tungstate solution (see below) 1.0 ml

Na2CO3 1.0 g

NaHCO3 7.6 g

NaCl 10.0 g

Yeast extract 0.2 g

Cellobiose 3.0 g

Na2S 9 H2O 0.5 g

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl2 4 H2O 1.5 g

ZnCl2 70.0 mg

MnCl2 4 H2O 100.0 mg

H3BO3 6.0 mg

CoCl2 6 H2O 190.0 mg

CuCl2 2 H2O 2.0 mg

NiCl2 6 H2O 24.0 mg

Na2MoO4 2 H2O 36.0 mg

Distilled water 990.0 ml

Selenite-tungstate solution:

NaOH 0.5 g

Na2SeO3 5 H2O 3 mg

Na2WO4 2 H2O 4 mg

Distilled water 1000.0 ml

Dissolve ingredients except cellobiose and sulfide, flush medium with N2 gas for 3060 min, dispense under N2 gas atmosphere and autoclave at 121C 15 min. Add cellobiose after autoclaving from an anoxic stock solution sterilized by filtration and sulfide from a sterile (autoclave at 121C 15 min), anoxic stock solution prepared under N2.

Final pH of the medium 8.8-9.0.

 

Updated 23.06.2021