235. Desulfonatronum cooperativum medium

KH2PO4 0.2 g

MgCl2 6 H2O 0.1 g

NH4Cl 1.0 g

KCl 0.2 g

Na2SO4 5.0 g

NaCl 10.0 g

Na2CO3 3.5 g

Trace element solution SL-10 (see below) 1.0 ml

Vitamin solution (see below) 10.0 ml

Yeast extract 1.0 g

Resazurin 0.5 mg

Na-formate 4.0 g

Na2S 9 H2O 0.5 g

Distilled water 1000.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl2 4 H2O 1.5 g

ZnCl2 70.0 mg

MnCl2 4 H2O 100.0 mg

H3BO3 6.0 mg

CoCl2 6 H2O 190.0 mg

CuCl2 2 H2O 2.0 mg

NiCl2 6 H2O 24.0 mg

Na2MoO4 2 H2O 36.0 mg

Distilled water 990.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine  10.0 mg

Riboflavin  5.0 mg

Pantotenoic acid 5.0 mg

p-Aminobenzoic acid 5.0 mg

Thiamine-HCl 5.0 mg

Nicotinic acid 5.0 mg

Vitamin B12 0.1 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

Dissolve the ingredients (except formate, vitamin solution and sulfide) and flush medium with 100 % N2 for 30 min. Add the sodium sulfide, adjust the pH to 8.8-9.0, dispense in Hungate tubes under N2, and autoclave at 121C 15 min. Before use add sodium formate and vitamin solution from a sterile, anaerobic stock solution (autoclave sodium formate 121C 15 min and vitamin solution by filtration).

 

Updated 23.06.2021