236. Desulfotomaculum carboxydivorans medium

Solution 1:

NaCl 1.17 g

MgCl₂ × 6 H₂O 0.4 g

KCl 0.3 g

CaCl₂ × 2 H₂O 0.15 g

NH₄Cl 0.27 g

KH₂PO₄ 0.2 g

Na₂SO₄ 2.84 g

Na-pyruvate 2.2 g

Yeast extract 0.5 g

Vitamin solution (see below) 1.0 ml

Trace element solution 1.0 ml (see below)

Distilled water 940.0 ml

Solution 2:

Na₂S × 9 H₂O 0.36 g

Distilled water 10.0 ml

Sodium bicarbonate for alkalization:

NaHCO₃ 4.5 g

Distilled water 50.0 g

Vitamin solution:

p-Aminobenzoic acid 4.0 mg

D(+)-Biotin 1.0 mg

Thiamine-HCl 10.0 mg

Distilled water 100.0 ml

Trace element solution:

FeCl₂ × 4 H₂O 1.5 g

ZnCl₂ 68.0 mg

MnCl₂ × 4 H₂O 100.0 mg

H₃BO₃ 62.0 mg

CoCl₂ × 6 H₂O 120.0 mg

Na₂MoO₄ × 2 H₂O 24.0 mg

HCl (0.05 M) 1000.0 ml

pH 7.0-7.2

Solution 1 is boiled before sterilization for a few minutes being flushed with gas mixture of 80% N₂ and 20% CO₂ with sodium bicarbonate added until an equilibrium pH of 6.9 - 7.1. Solution 1 is autoclaved under this gas mixture at 121°C for 15 min. Solution 2 is autoclaved under 100% N₂ at 121°C for 15 min. Vitamin solution and Na-pyruvate is filter sterilized.

 

Updated 1.02.2024