238. Aquaspirillum medium II

(NH4)2SO4 1.0 g

MgSO4 7 H2O 1.0 g

CaCl2 2 H2O 30.0 mg

Na2HPO4 10.0 mg

Casamino acids 1.5 g

Sodium succinate (10% solution) 10.0 ml

Na2S2O3 5 H2O (10% solution) 1.0 ml

Vitamin solution (see below) 5.0 ml

Trace element solution SL-10 (see below) 1.0 ml

Vitamin solution:

Biotin 200.0 mg

Folic acid 20.0 mg

Pyridoxine-HCl 100.0 mg

Thiamine-HCl 50.0 mg

Riboflavin 100.0 mg

Nicotinic acid 50.0 mg

DL-Pantothenic acid 50.0 mg

Vitamin B12 1.0 mg

p-Aminobenzoic acid 50.0 mg

Distilled water 1000.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl3 4 H2O 1.5 g

ZnCl2 70.0 mg

MnCl2 4 H2O 100.0 mg

H3BO3 6.0 mg

CoCl2 6 H2O 190.0 mg

CuCl2 2 H2O 2.0 mg

NiCl2 6 H2O 24.0 mg

Na2MoO4 2 H2O 36.0 mg

Distilled water 990.0 ml

Agar 0.5 g

Distilled water 985.0 ml

pH 7.5

Basal medium, trace element solution autoclave separately at 121C 15 min. Vitamin solution sterilize by filtration.

 

Updated 30/05/2020