238. Aquaspirillum medium II

(NH₄)₂SO₄ 1.0 g

MgSO₄ × 7 H₂O 1.0 g

CaCl₂ × 2 H₂O 30.0 mg

Na₂HPO₄ 10.0 mg

Casamino acids 1.5 g

Sodium succinate (10% solution) 10.0 ml

Na₂S₂O₃ × 5 H₂O (10% solution) 1.0 ml

Vitamin solution (see below) 5.0 ml

Trace element solution SL-10 (see below) 1.0 ml

Vitamin solution:

Biotin 200.0 mg

Folic acid 20.0 mg

Pyridoxine-HCl 100.0 mg

Thiamine-HCl 50.0 mg

Riboflavin 100.0 mg

Nicotinic acid 50.0 mg

DL-Pantothenic acid 50.0 mg

Vitamin B₁₂ 1.0 mg

p-Aminobenzoic acid 50.0 mg

Distilled water 1000.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl₂ × 4 H₂O 1.5 g

ZnCl₂ 70.0 mg

MnCl₂ × 4 H₂O 100.0 mg

H₃BO₃ 6.0 mg

CoCl₂ × 6 H₂O 190.0 mg

CuCl₂ × 2 H₂O 2.0 mg

NiCl₂ × 6 H₂O 24.0 mg

Na₂MoO₄ × 2 H₂O 36.0 mg

Distilled water 990.0 ml

Agar 0.5 g

Distilled water 985.0 ml

pH 7.5

Basal medium, trace element solution autoclave separately at 121°C 15 min. Vitamin solution sterilize by filtration.

 

Updated 1.02.2024