247. RHODOBLASTUS MEDIUM

Yeast extract 0.1 g

Na₂-succinate 1.0 g

KH₂PO₄ 0.5 g

MgSO₄ × 7 H₂O 0.4 g

NaCl 0.4 g

NH₄Cl 0.4 g

CaCl₂ × 2 H₂O 0.05 g

Trace element solution SL-6 (see below) 1.0 ml

Methanol 0.01-1%

Distilled water 1000.0 ml

pH 5.7

Trace element solution SL-6:

ZnSO₄ × 7 H₂O 0.1 g

MnCl₂ × 4 H₂O 0.03 g

H₃BO₃ 0.3 g

CoCl₂ × 6 H₂O 0.2 g

CuCl₂ × 2 H₂O 0.01 g

NiCl₂ × 6 H₂O 0.02 g

Na₂MoO₄ × 2 H₂O 0.03 g

Distilled water 1000.0 ml

Autoclave basal medium and trace element solution separately at 121°C 15 min, methanol sterilize by filtration.

 

Updated 1.02.2024