248. PFENNIG'S MEDIUM

KH2PO4 0.34 g

NH4Cl 0.34 g

MgSO4 7 H2O 0.5 g

CaCl2 2 H2O 0.05 g

KCl 0.34 g

Trace element solution SLA (see below) 1.0 ml

Vitamin B12 20 g

NaHCO3 1.5 g

Na2S 9 H2O 0.4 g

Na2S2O3 5 H2O 0.5 g

NaCl 15.0 g

MgCl2 6 H2O 2.5 g.

pH 7.5

Trace element solution SLA:

FeCl2 4 H2O 1.8 mg

CoCl2 6 H2O 250 mg

NiCl2 6 H2O 10 mg

CuCl2 2 H2O 10 mg

MnCl2 4 H2O 70 mg

ZnCl2 100 mg

H3BO3 500 mg

Na2MoO4 2 H2O 30 mg

Na2SeO3 5 H2O 10 mg

Distilled water 1000.0 ml

Autoclave basal medium and trace element solution separately at 121C 15 min, vitamin B12 sterilize by filtration.

 

Updated 25.06.2021