250. MODIFIED MEDIUM FOR MARINE METHYLOTROPHIC BACTERIA WITH Yeast extract AND Biotin

KH₂PO₄ 2.0 g

(NH₄)₂SO₄ 2.0 g

MgSO₄ × 7 H₂O 0.025 g

FeSO₄ × 7 H₂O 0.02 g

Yeast extract 0.1 g

Methanol 10.0 ml

Biotin 0.01 mg

Distilled water 1000.0 ml

pH 7.0

Autoclave at 121°C 15 min, methanol and biotin sterilize by filtration.

 

Updated 1.02.2024