253. MODIFIED MEDIUM FOR METHANOTROPHIC BACTERIA WITH ETHANOL

KNO₃ 1.0 g

KH₂PO₄ 0.7 g

MgSO₄ × 7 H₂O 0.2g

CaCl₂ × 2 H₂O 0.02 g

Na₂HPO₄ × 2 H₂O 1.5 g

Na₂-EDTA 5.0 mg

FeSO₄ × 7 H₂O 2.0 mg

ZnSO₄ × 7 H₂O 0.1 mg

MnCl₂ × 4 H₂O 0.03 mg

CoCl₂ × 6 H₂O 0.2 mg

CuCl₂ × 2 H₂O 0.1 mg

NiCl₂ × 6 H₂O 0.02 mg

Na₂MoO₄ × 2 H₂O 0.03 mg

Ethanol 5.0 ml

Distilled water 1000.0 ml

pH 6.7 - 7.1

Autoclave at 121°C 15 min, ethanol sterilize by filtration.

Cultivation under mixture of methane and air (1:1).

 

Updated 1.02.2024