256. MODIFIED METHYLOTROPH MEDIUM 1 WITH YEAST AUTOLYZATE

KH₂PO₄ 2.0 g

(NH₄)₂SO₄ 2.0 g

MgSO₄ × 7 H₂O 0.025 g

NaCl 0.5 g

FeSO₄ × 7 H₂O 0.02 g

Methanol 5.0 ml or Methylamine 3.0 g

Yeast autolizate 1.0 g

Distilled water 1000.0 ml

pH 7.0

Autoclave at 121°C 15 min, methanol and methylamine sterilize by filtration.

 

Updated 1.02.2024