266. ACIDOSOMA MEDIUM

KH₂PO₄ 0.1 g

(NH₄)₂SO₄ 0.25 g

CaCl₂ × 2 H₂O 0.05 g

MgSO₄ × 7 H₂O 0.02 g

Trace elements (see below) 1.00 ml

Yeast extract 0.10 g

Na gluconate 0.50 g

Distilled water 1000.00 ml

Trace elements:

EDTA 5.00 g

CuCl₂ × 2 H₂O 0.10 g

FeSO₄ × 7 H₂O 2.00 g

ZnSO₄ × 7 H₂O 0.10 g

NiCl₂ × 6 H₂O 0.02 g

CoCl₂ × 6 H₂O 0.20 g

Na₂MoO₄ 0.03 g

Distilled water 1000.00 ml

Autoclave the trace base medium and element solution separately at 121°C for 15 min

Adjust to pH 5.0 – 5.5. Agar may be added at 15 g/l for solid media.