268. ZAVARZINELLA FORMOSA MEDIUM

KH₂PO₄ 0.1 g

MgSO₄ × 7 H₂O 0.1 g

CaCl₂ × 6 H₂O 0.05 g

NaCl 0.01 g

N-acetylglucosamine 1.0 g

Glucose 0.5 g

Peptone 0.1 g

Yeast extract 0.1 g

Casamino acids 0.1 g

Metal solution “44” (see below) 1.0 ml

Agar (for solid media) 15.0 g

Distilled water 1000.0 ml

Final pH 5.8–6.0

Metal solution “44”:

Na-EDTA 250.0 mg

ZnSO₄ × 7 H₂O 1095.0 mg

FeSO₄ × 7 H₂O 500.0 mg

MnSO₄ × 7 H₂O 154.0 mg

CuSO₄ × 5 H₂O 39.0 mg

Co(NO3)₂ × 6 H₂O 24.8 mg

Na₂B₄O₇ × 10 H₂O 17.7 mg

Distilled water 1000.0 ml

Dissolve Na-EDTA and add a few drops of concentrated H₂SO₄ to retard precipitation of heavy metal ions.

Autoclave at 121°C for 15 min.