272. ECTOTHIORHODOSPIRA MAGNA MEDIUM

KH2PO4 0.5 g

NaCl 30.0 g

NH4Cl 0.5 g

MgCl2 × 6 H2O 0.2 g

Na-acetate 1.0 g

Na2S2O3 0.5 g

Na2S × 9 H2O 0.5 g

NaHCO3 5.0 g

Na2CO3 5.0 g

Yeast extract 0.1 g

Vitamin B12 10.0 mkg

Trace elements solution (see below) 1.0 ml

Distilled water 1000.0 ml

pH 9.0-9.5

Prepare the medium without the NaHCO3, Na2S × 9 H2O, Na2CO3 and vitamin B12, under a nitrogen atmosphere.

Autoclave at 121°C for 15 min and add the vitamin B12 from a filter-sterilized stock solution and the NaHCO3 (autoclaved in sealed, half full vessels), Na2S × 9 H2O, Na2CO3 from autoclaved at 121°C for 15 min stock solutions. The final pH of the medium should be 9.0-9.5.

Trace elements solution:

EDTA 5.0 g

FeSO4 × 7 H2O 2.2 g

ZnSO4 × 7 H2O 0.1 g

MnCl2 × 4 H2O 0.03 g

H3BO3 0.03 g

CoCl2 × 6 H2O 0.2 g

CuCl2 × 2 H2O 0.03 g

NiCl2 × 6 H2O 0.03 g

Na2MoO4 × 2 H2O 0.03 g

Distilled water 1000.0 ml

pH 3.0-4.0

 

Updated 02.08.2021