280. PLANCTOMYCES MEDIUM

KH2PO4 0.1 g

(NH4)2SO4 0.2 g

MgSO4 × 7 H2O 0.1 g

CaCl2 × 6 H2O 0.02 g

NaCl 0.01 g

N-acetylglucosamine 1.0 g

Glucose 0.5 g

Yeast e×tract 0.2 g

Hutner's basal salts (see below) 1.0 ml

Staley's vitamin solution (see below) 1.0 ml

Phytagel (for solid medium) 8.0 g

Distilled water 1000.0 ml

Adjust pH to 5.8-6.5

Autoclave at 121°C 15 min. Add filter-sterilized vitamin solution

Hutner's basal salts:

Nitrilotriacetic acid (NTA) 10.0 g

MgSO4 × 7 H2O 29.7 g

CaCl2 × 2 H2O 3.34 g

Na2MoO4 × 2 H2O 12.67 mg

FeSO4 × 7 H2O 99.0 mg

Metal salt solution "44" (see below) 50 ml

Dissolve NTA first by neutralizing with KOH, then add other salts.

pH 7.2 (ajust with KOH or H2SO4).

Ajust volume to 1000.0 ml with distilled water.

Metal salt solution "44":

Na-EDTA 250.0 mg

ZnSO4 × 7 H2O 1095.0 mg

FeSO4 × 7 H2O 500.0 mg

MnSO4 × 7 H2O 154.0 mg

CuSO4 × 5 H2O 39.0 mg

Co(NO3)2 × 6 H2O 24.8 mg

Na2B4O7 × 10 H2O 17.7 mg

Distilled water 1000.0 ml

Dissolve Na-EDTA and add a few drops of concentrated H2SO4 to retard precipitation of heavy metal ions.

Staley’s vitamin solution:

Vitamin B12 0.10 mg

Biotin 2.0 mg

Thiamine-HCl × 2 H2O 5.0 mg

Ca-pantothenate 5.0 mg

Folic acid 2.0 mg

Riboflavin 5.0 mg

Nicotinamide 5.0 mg

p-Aminobenzoic acid 5.0 mg

Pyridoxine-HCl 10.0 mg

Distilled water 1000.0 ml

Store in the dark and cold (5°C).

Updated 25.06.2021