281. AZOSPIRILLUM AMAZONENSE MEDIUM

K₂HPO₄ 0.2 g

KH₂PO₄ 0.6 g

CaCl₂ × 2 H₂O 0.02 g

MgSO₄ × 7 H₂O 0.2 g

Na₂MoO₄ × 2 H₂O 0.002 g

FeCl₂ × 2 H₂O 0.01 g

Bromothymol blue (0.5% in 0.2N KOH) 5.0 ml

Sucrose 5.0 g

Distilled water 1000.0 ml

Adjust pH to 6.0

For semisolid medium, add 0.5 g of agar; for solid medium, add 15 g of agar.

Autoclave at 105°C for 30 min.