283. AZOSPIRILLUM VM medium

Dobereiner basic 10 ml

Fe₃ EDTA 0.66% (w/v) 10.0 ml

NH₄Cl 0.5 g

NaCl 1.0 g

Yeast extract 1.0 g

Peptone 3.0 g

Phosphate buffer pH 6.8 3.0 ml

Agar 15.0 g

Distilled water 1000.0 ml

Dobereiner's basic:

MgSO₄ 20.0 g

NaCl 10.0 g

CaCl₂ 2.64 g

Na₂MoO₄ × 2H₂O 0.2 g

MnSO₄ × 2H₂O 1.0 g

Distilled water 1000.0 ml

Phosphate buffer pH 6.8:

KH₂PO₄ 0.6 g

K₂HPO₄ 0.4 g

Distilled water 3 ml

Autoclave basal medium, Fe₃ EDTA solution, Dobereiner basic and phosphate buffer separately at 121°C for 15 min.