287. METHYLOCAPSA AUREA MEDIUM

NaNO3 200.0 mg

MgSO4 x 6 H2O 200.0 mg

CaCl2 x 2 H2O 40.0 mg

FeEDTA solution 3.0 ml

100mM Phosphate buffer pH 5.8 10.0 ml

Trace elements solution (see below)1.0 ml

Distilled water 986.0 ml

Trace elements solution:

EDTA 5.0 g

CuCl2 x 5 H2O 0.1 g

FeSO4 x 7 H2O 2.0 g

ZnSO4 x 7 H2O 0.1 g

NiCl2 x 6 H2O 0.02 g

CoCl2 x 6 H2O 0.2 g

Na2MoO4 0.03 g

Distilled water 1000.0 ml

FeEDTA solution:

FeSO4 x 7 H2O 1.54 g

Na2EDTA 2.06 g

Distilled water 1000.0 ml

100mM Phosphate buffer:

K2HPO4 x 3 H2O 1.71 g

KH2PO4 12.58 g

Distilled water 1000.0 ml

Autoclave at 121C for 15 min. Final pH 5.0-5.8. 20-30% methane is added to the gas phase. The strain should be grown with shaking.

Updated 20/05/2020