301. METHYLOFERULA STELLATA MEDIUM

KH2PO4 100.0 mg

(NH4)2SO4 100.0 mg

MgSO4 × 7 H2O 50.0 mg

CaCl2 × 2 H2O 10.0 mg

NaCl 20.0 mg

FeEDTA solution 3.0 ml

Trace elements solution (see below) 1.0 ml

Methanol 10.0 ml

Distilled water 1000.0 ml

Trace elements solution:

EDTA 5.0 g

CuCl2 × 2 H2O 0.1 g

FeSO4 × 7 H2O 2.0 g

ZnSO4 × 7 H2O 0.1 g

NiCl2 × 6 H2O 0.02 g

CoCl2 × 6 H2O 0.2 g

Na2MoO4 0.03 g

Distilled water 1000.0 ml

FeEDTA solution:

FeSO2 × 7 H2O 1.54 g

Na2EDTA 2.06 g

Distilled water 1000.0 ml

Final pH 5:0-5.8.

The medium is fairly weakly buffered so the pH should be checked before and after autoclaving at 121°C for 15 min. Methanol is filter sterilized. The pH should be adjusted with H3PO4 (sterilised if added to sterile medium). The strain should be grown with shaking.

Updated 29.06.2021