332. THERMOGUTTA MEDIUM

KH2PO4 0.33 g

MgCl2 6 H2O 0.33 g

CaCl2 2 H2O 0.33 g

NH4Cl 0.33 g

KCl 0.33 g

NaCl 6.0

Yeast extract 0.2 g

Xanthan 5.0 g

KNO3 2.0 g

NaHCO3 2.0 g

Trace element solution (see below) 1.0 ml

Vitamin solution (see below) 1.0 ml

Trace element solution:

Fe(NH4)2(SO4)2 6 H2O (Mohr's salt) 784.0 mg

CoCl2 H2O 238.0 mg

Ni(NH4)2(SO4)2 6 H2O 395.0 mg

Na2MoO4 2 H2O 24.0 mg

Na2WO4 2 H2O 33.0 mg

ZnSO4 7 H2O 144.0 mg

CuCl2 2 H2O 2.0 mg

Na2SeO4 94.0 mg

H3BO3 6.0 mg

MnCl2 4 H2O 99.0 mg

Mohr's salt is dissolved firstly in concentrated HCl, and then is mixed with water and other salts are dissolved in the sequence indicated.

Vitamin solution:

Biotin 20.0 mg

Folic acid 20.0 mg

Pyridoxine 10.0 mg

Riboflavin  50.0 mg

Pantotenoic acid 50.0 mg

p-Aminobenzoic acid 50.0 mg

Thiamine-HCl 50.0 mg

Nicotinic acid 50.0 mg

Vitlamin B12 1.0 mg

Lipoic acid 50.0 mg

Distilled water 1000.0 ml

The media was boiled and cooled under the flow of oxygen-free gases (CO2 or N2) to render the media anaerobic and finally heat-sterilized at 121C for 60 min. No reducing agents were added.

Updated 09.07.2021