337. CUNICULIPLASMA MEDIUM

Beef extract 3 g

Betaine 0.6 g

(NH4)2SO4 1.3 g

KH2PO4 0.28 g

MgSO4 7 H2O 0.25 g

CaCl2 2 H2O 0.07 g

FeCl3 6 H2O 0.02 g

Trace element solution SL-10 (see below) 1.0 ml

Vitamin solution (see above) 10.0 ml

Distilled water 990.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl2 4 H2O 1.5 g

ZnCl2 70.0 mg

MnCl2 4 H2O 100.0 mg

H3BO3 6.0 mg

CoCl2 6 H2O 190.0 mg

CuCl2 2 H2O 2.0 mg

NiCl2 6 H2O 24.0 mg

Na2MoO4 2 H2O 36.0 mg

Distilled water 990.0 ml

Vitamin solution:

myo-Inositol 1.0 g

Thiamine-HCl 100.0 mg

Pyridoxine-HCl 100.0 mg

Folic acid 40.0 mg

D-Biotin 10.0 mg

L-Ascorbic acid 200.0 mg

D-Ca-pantothenate 100.0 mg

Choline chloride 100.0 mg

Nicotinamide 100.0 mg

Riboflavin 20.0 mg

Vitamin B12 20.0 mg

Vitamin A 10.0 mg

p-Aminobenzoic acid 20.0 mg

Distilled water 990.0 ml

 

The medium was adjusted to pH 1.01.2 with concentrated H2SO4. The cultures were incubated for about 5 days at 37oC with shaking.

 

Updated 09.07.2021