337. CUNICULIPLASMA MEDIUM

Beef extract 3 g

Betaine 0.6 g

(NH₄)₂SO₄ 1.3 g

KH₂PO₄ 0.28 g

MgSO₄ × 7 H₂O 0.25 g

CaCl₂ × 2 H₂O 0.07 g

FeCl₃ × 6 H₂O 0.02 g

Trace element solution SL-10 (see below) 1.0 ml

Vitamin solution (see above) 10.0 ml

Distilled water 990.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl₂ × 4 H₂O 1.5 g

ZnCl₂ 70.0 mg

MnCl₂ × 4 H₂O 100.0 mg

H₃BO₃ 6.0 mg

CoCl₂ × 6 H₂O 190.0 mg

CuCl₂ × 2 H₂O 2.0 mg

NiCl₂ × 6 H₂O 24.0 mg

Na₂MoO₄ × 2 H₂O 36.0 mg

Distilled water 990.0 ml

Vitamin solution:

myo-Inositol 1.0 g

Thiamine-HCl 100.0 mg

Pyridoxine-HCl 100.0 mg

Folic acid 40.0 mg

D-Biotin 10.0 mg

L-Ascorbic acid 200.0 mg

D-Ca-pantothenate 100.0 mg

Choline chloride 100.0 mg

Nicotinamide 100.0 mg

Riboflavin 20.0 mg

Vitamin B₁₂ 20.0 mg

Vitamin A 10.0 mg

p-Aminobenzoic acid 20.0 mg

Distilled water 990.0 ml

 

The medium was adjusted to pH 1.0–1.2 with concentrated H₂SO₄. The cultures were incubated for about 5 days at 37^oC with shaking.

 

Updated 01.02.2024