340. DISSULFURIMICROBIUM MEDIUM

KH2PO4 0.33 g

NH4Cl 0.33 g

KCl 0.33 g

CaCl2 2 H2O 0.33 g

MgCl2 6 H2O, 0.33 g

NaHCO3 2.0 g

Vitamin solution (see below) 10.0 ml

Trace element solution (see below) 1.0 ml

Ferrihydrite

Distilled water 990.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine (B2) 10.0 mg

Riboflavin (B1) 5.0 mg

Pantotenoic acid 5.0 mg

p-Aminobenzoic acid 5.0 mg

Thiamine-HCl 5.0 mg

Nicotinic acid 5.0 mg

Vitamin B12 0.1 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

pH 7.2-7.4

Trace element solution:

Na2-EDTA 2 H2O 5.2 g

FeCl2 4 H2O 1.5 g

ZnCl2 70.0 mg

MnCl2 4 H2O 100.0 mg

H3BO3 6.0 mg

CoCl2 6 H2O 190.0 mg

CuCl2 2 H2O 2.0 mg

NiCl2 6 H2O 24.0 mg

Na2MoO4 2 H2O 36.0 mg

Distilled water 1000.0 ml

Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000.0 ml. The pH of the autoclaved medium was 6.76.8. Medium (10.0 ml) was dispensed into 17 ml Hungate tubes; the headspace was filled with CO2 (100 %).

 

Updated 11.08.2021