345. Natronospirillum sperandus medium

NaCl 34.0 g

NaHCO₃ 20.0 g

K₂HPO₄ 0.3 g

MgCl₂ × 6 H₂O 0.12 g

CaCl₂ × 2 H₂O 0.03 g

Casamino acids 3.0 g

Yeast extract 0.1 g

Agar 20.0 g

Trace element solution (see below) 10.0 ml

Distilled water 1000.0 ml. Sterilize at 121°C for 15 min.

Final pH 9.0 – 9.5

Trace element solution:

FeSO₄ × 7 H₂O 0.6 mg

CoCl₂ × 6 H₂O 0.12 mg

NiSO₄(NH₄)₂SO₄ × 6 H₂O 0.9 mg

MnCl₂ × 4 H₂O 0.1 mg

ZnSO₄ × 7 H₂O 0.14 mg

H₃BO₃ 0.03 mg

AlK(SO₄)₂ × 12 H₂O 0.24 mg

Na₂MoO₄ × 2 H₂O 0.02 mg

Na₂WO₄ × 2 H₂O 0.03 mg

Na₂SeO₃ 0.02 mg

CuCl₂ × 2 H₂O 0.02 mg

Distilled water 1000.0 ml

Autoclave the trace element solution and NaHCO₃ separately at 121°C for 15 min and add to the medium.

Final pH 8.7 – 8.9

 

Updated 04.08.2021