347. Succinate minimal salt medium (SMS)

EDTA 0.01 g

KH2PO4 0.6 g

K2HPO4 0.9 g

NH4Cl 1.0 g

MgSO4 × 7 H2O 0.2 g

CaCl2 × 6 H2O 0.075 g

Na-succinate 2.2 g

Yeast extract 0.1 g

Trace elements solution (see below) 2.0 ml

Vitamin solution (see below) 2.0 ml

Distilled water 1000.0 ml

Trace elements solution:

FeSO4 × 7 H2O 300.0 mg

ZnSO4 × 7 H2O 5.0 mg

MnCl2 × 4 H2O 3.0 mg

H3BO3 2.0 mg

CoCl2 × 6 H2O 5.0 mg

CuCl2 × 2 H2O 1.0 mg

NiCl2 × 6 H2O 2.0 mg

Na2MoO4 × 2 H2O 3.0 mg

Distilled water 1000.0 ml

Vitamin solution:

Biotin 80.0 mg

Thiamine-HCl × 2 H2O 400.0 mg

Nicotinic acid 400.0 mg

Vitamin B12 20.0 mg

Distilled water 1000.0 ml

Adjust pH to 6.8. Prepare the medium without the vitamin solution. Autoclave at 121°C for 15 min and add the vitamin solution from a filter-sterilised stock solution.

 

Updated 09.07.2021