347. Succinate minimal salt medium (SMS)

EDTA 0.01 g

KH2PO4 0.6 g

K2HPO4 0.9 g

NH4Cl 1.0 g

MgSO4 7 H2O 0.2 g

CaCl2 6 H2O 0.075 g

Na-succinate 2.2 g

Yeast extract 0.1 g

Trace elements solution (see below) 2.0 ml

Vitamin solution (see below) 2.0 ml

Distilled water 1000.0 ml

Trace elements solution:

FeSO4 7 H2O 300.0 mg

ZnSO4 7 H2O 5.0 mg

MnCl2 4 H2O 3.0 mg

H3BO3 2.0 mg

CoCl2 6 H2O 5.0 mg

CuCl2 2 H2O 1.0 mg

NiCl2 6 H2O 2.0 mg

Na2MoO4 2 H2O 3.0 mg

Distilled water 1000.0 ml

Vitamin solution:

Biotin 80.0 mg

Thiamine-HCl 2 H2O 400.0 mg

Nicotinic acid 400.0 mg

Vitamin B12 20.0 mg

Distilled water 1000.0 ml

Adjust pH to 6.8. Prepare the medium without the vitamin solution. Autoclave at 121C for 15 min and add the vitamin solution from a filter-sterilised stock solution.

 

Updated 09.07.2021