347. Succinate minimal salt medium (SMS)
EDTA 0.01 g
KH2PO4 0.6 g
K2HPO4 0.9 g
NH4Cl 1.0 g
MgSO4 × 7 H2O 0.2 g
CaCl2 × 6 H2O 0.075 g
Na-succinate 2.2 g
Yeast extract 0.1 g
Trace elements solution (see below) 2.0 ml
Vitamin solution (see below) 2.0 ml
Distilled water 1000.0 ml
Trace elements solution:
FeSO4 × 7 H2O 300.0 mg
ZnSO4 × 7 H2O 5.0 mg
MnCl2 × 4 H2O 3.0 mg
H3BO3 2.0 mg
CoCl2 × 6 H2O 5.0 mg
CuCl2 × 2 H2O 1.0 mg
NiCl2 × 6 H2O 2.0 mg
Na2MoO4 × 2 H2O 3.0 mg
Distilled water 1000.0 ml
Vitamin solution:
Biotin 80.0 mg
Thiamine-HCl × 2 H2O 400.0 mg
Nicotinic acid 400.0 mg
Vitamin B12 20.0 mg
Distilled water 1000.0 ml
Adjust pH to 6.8. Prepare the medium without the vitamin solution. Autoclave at 121°C for 15 min and add the vitamin solution from a filter-sterilised stock solution.
Updated 09.07.2021