348. RO (RICH ORGANIC) MEDIUM
MgSO4 × 7 H2O 0.3 g
KH2PO4 0.3 g
NH4Cl 0.1 g
CaCl2 × 2 H2O 1.0 g
Na-acetate × 3 H2O 1.0 g
Yeast Extract 0.5 g
Peptone 0.5 g
Casamino аcids 0.5 g
Trace elements solution (see below) 2.0 ml
Vitamin solution (see below) 2.0 ml
Distilled water 1000.0 ml
Trace elements solution:
FeSO4 × 7 H2O 300.0 mg
ZnSO4 × 7 H2O 5.0 mg
MnCl2 × 4 H2O 3.0 mg
H3BO3 2.0 mg
CoCl2 × 6 H2O 5.0 mg
CuCl2 × 2 H2O 1.0 mg
NiCl2 × 6 H2O 2.0 mg
Na2MoO4 × 2 H2O 3.0 mg
Distilled water 1000.0 ml
Vitamin solution:
Biotin 80.0 mg
Thiamine-HCl × 2 H2O 400.0 mg
Nicotinic acid 400.0 mg
Vitamin B12 20.0 mg
Distilled water 1000.0 ml
Autoclave base medium, KH2PO4 (in 10.0 ml distilled water) and trace element solution at 121°C for 15 min. Vitamin solution sterilize by filtration.
Updated 1.02.2024