348. RO (RICH ORGANIC) MEDIUM

MgSO× 7 H2O 0.3 g

KH2PO4 0.3 g

NH4Cl 0.1 g

CaCl2 × 2 H2O 1.0 g

Na-acetate × 3 H2O 1.0 g

Yeast Extract 0.5 g

Peptone 0.5 g

Casamino аcids 0.5 g

Trace elements solution (see below) 2.0 ml

Vitamin solution (see below) 2.0 ml

Distilled water 1000.0 ml

Trace elements solution:

FeSO4 × 7 H2O 300.0 mg

ZnSO4 × 7 H2O 5.0 mg

MnCl2 × 4 H2O 3.0 mg

H3BO3 2.0 mg

CoCl2 × 6 H2O 5.0 mg

CuCl2 × 2 H2O 1.0 mg

NiCl2 × 6 H2O 2.0 mg

Na2MoO4 × 2 H2O 3.0 mg

Distilled water 1000.0 ml

Vitamin solution:

Biotin 80.0 mg

Thiamine-HCl × 2 H2O 400.0 mg

Nicotinic acid 400.0 mg

Vitamin B12 20.0 mg

Distilled water 1000.0 ml

Autoclave base medium, KH2PO4 (in 10.0 ml distilled water) and trace element solution at 121°C for 15 min. Vitamin solution sterilize by filtration.

 

Updated 1.02.2024