350. THERMOGEMMATA POLYSACCHARIDOLYTICA MEDIUM

KH2PO4 0.165 g

MgCl2 × 6 H2O 0. 165 g

CaCl2 × 2 H2O 0. 165 g

NH4Cl 0. 165 g

KCl 0. 165 g

Maltose or trehalose 1.0 g

Trace element solution (see below) 1.0 ml

Vitamin solution (see below) 1.0 ml

Distilled water 1000.0 ml

Trace element solution:

(NH4)2Fe(SO4)2 × 6 H2O (Mohr's salt) 784.0 mg

HCl (concentrated) 5.0 ml

CoCl2 × H2O 238.0 mg

(NH4)2Ni(SO4)2 × 6 H2O 395.0 mg

Na2MoO4 × 2 H2O 24.0 mg

Na2WO4 × 2 H2O 33.0 mg

ZnSO4 × 7 H2O 144.0 mg

CuCl2 × 2 H2O 2.0 mg

Na2SeO4 94.0 mg

H3BO3 6.0 mg

MnCl2 × 4 H2O 99.0 mg

Distilled water 995.0 ml

Mohr's salt is dissolved firstly in concentrated HCl, then is mixed with distilled water and other salts are dissolved in the sequence indicated.

Vitamin solution:

Biotin 20.0 mg

Folic acid 20.0 mg

Pyridoxine (B2) 100.0 mg

Riboflavin (B1) 50.0 mg

Pantotenoic acid 50.0 mg

p-Aminobenzoic acid 50.0 mg

Thiamine-HCl 50.0 mg

Nicotinic acid 50.0 mg

Vitamin B12 1.0 mg

Lipoic acid 50.0 mg

Distilled water 1000.0 ml

Autoclave base medium, KH2PO4 (in 10.0 ml distilled water) and trace element solution at 121°C for 15 min. Vitamin solution sterilize by filtration. Adjust pH to 7.0-7.5.

 

Updated 1.02.2024