354. BACTO MARINE BROTH (DIFCO 2216)

Bacto peptone 5.0 g

Bacto yeast extract 1.0 g

Fe(III) citrate 0.1 g

NaCl 80.0 g

MgCl2 (anhydrous) 5.9 g

Na2SO4 3.24 g

CaCl2 2 H2O 1.8 g

KCl 0.55 g

NaHCO3 0.16 g

KBr 0.08 g

SrCl2 34.0 mg

H3BO3 22.0 mg

Na-silicate 4.0 mg

NaF 2.4 mg

NH4NO3 1.6 mg

Na2HPO4 8.0 mg

Distilled water 1000.0 ml C.

Final pH should be 7.6 0.2 at 25 C

If using the complete medium from Difco add 37.4 g per liter water + 6% NaCl.

 

Updated 11.07.2021