361. METHANOBACTERIUM ARCTICUM MEDIUM

Solution 1:

KCl 0.34 g

MgCl₂ × 7 H₂O 0.4 g

MgSO₄ × 7 H₂O 0.35 g

CaCl₂ × 2 H₂O 0.1 g

NH₄Cl 0.25 g

KH₂ PO₄ 0.14 g

NaCl 5.0 g

Resazurin 0.01 g

NaHCO₃ 5.0 g

Trace element solution (see below) 10.0 ml

Distilled water 965.0 ml

Solution 2 (reducing agents):

L-Cysteine-HCl chloride × H₂O 0.5 g

Na₂S × 9 H₂O 0. 5 g

Distilled water 10.0 ml

Trace element solution:

Nitrilotriacetic acid 1.5 g

MgSO₄ × 7 H₂O 3.0 g

MnSO₄ × 7 H₂O 0.5 g

NaCl 1.0 g

FeSO₄ × 7 H₂O 0.1 g

CoSO₄ 0.18 g

CaCl₂ × 2 H₂O 0.1 g

ZnSO₄ 0.18 g

CuSO₄ 0.01 g

KAl(SO₄)₂ × 12 H₂O 0.02 g

H₃BO₃ 0.01 g

Na₂MoO₄ × 2 H₂O 0.01 g

NiCl₂ × 6 H₂O 0.025 g

Na₂SeO₃ × 5 H₂O 0.3 mg

Distilled water 1000.0 ml

pH 7.0-7.4.

 

Updated 11.07.2021