371. MINERAL MEDIUM “P” FOR METHANOTROPHIC BACTERIA

Na2HPO4 × 12 H2O 1.5 g

KH2PO4 0.7 g

KNO3 1.0 g

MgSO4 × 7 H2O 0.2 g

CaCl2 × 2 H2O 0.02 g

NaCl 30.0 g

NaHCO3 4.2 g

Na2CO3 0.05 g

Trace element solution (see below) 1.0 ml

Distilled water 1000.0 ml

Trace elements solution:

EDTA 5.0 g

CuCl2 × 2 H2O 0.1 g

FeSO4 × 7 H2O 2.0 g

ZnSO4 × 7 H2O 0.1 g

NiCl2 × 6 H2O 0.02 g

CoCl2 × 6 H2O 0.2 g

Na2MoO4 0.03 g

Distilled water 1000.0 ml

Prepare the medium without the NaHCO3 and Na2CO3. Basal medium, trace element solution, NaHCO3 and Na2CO3 sterilize separately at 121°C for 15 min. When preparing liquid media cool the mineral salts solution, NaHCO3 and Na2CO3 to room temperature before mixing. When preparing agar add 2.0 % agar to the mineral salt solution and autoclave. Cool the NaHCO3 and Na2CO3 stock solution and agar to 50-55°C before mixing.

The pH of the complete medium should be at 9.0.

 

Updated 31/05/2020