373. MARINE AMMONIUM MINERAL SALTS

Solution A:

NaCl 20.0 g

(NH₄)₂SO₄ 1.0 g

CaCl₂ × 2 H₂O 0.2 g

MgSO₄ × 7 H₂O 1.0 g

FeSO₄ × 7 H₂O 0.02 g

Na₂WO4 × 2 H₂O 0.03 g

Na₂MoO₄ × 2 H₂O 0.02 g

Methanol 5.0 ml

Trace element solution SL-10 (see below) 1.0 ml

Vitamin solution (see below) 1.0 ml

Distilled water 900.0 ml

Adjust the pH to 7.2 if needed with NaOH/HCl

Solution B:

KH₂PO₄ 0.36 g

K₂HPO₄ 2.34 g

Distilled water 100.0 ml

Trace element solution SL-10:

HCl (25%; 7.7 M) 10.0 ml

FeCl₂ × 4 H₂O 1.5 g

ZnCl₂ 70.0 mg

MnCl₂ × 4 H₂O 100.0 mg

H₃BO₃ 6.0 mg

CoCl₂ × 6 H₂O 190.0 mg

CuCl₂ × 2 H₂O 2.0 mg

NiCl₂ × 6 H₂O 24.0 mg

Na₂MoO₄ × 2 H₂O 36.0 mg

Distilled water 990.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine 10.0 mg

Riboflavin 5.0 mg

Pantotenoic acid 5.0 mg

p-Aminobenzoic acid 5.0 mg

Thiamine-HCl 5.0 mg

Nicotinic acid 5.0 mg

Vitamin B₁₂ 0.1 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

Autoclave solution A, B and trace element solution separately at 121°C for 15 min and combine after cooling. Add filter sterilize methanol and vitamin solution.

 

Updated 12.07.2021