375. Methylomicrobium buryatense MEDIUM

KH2PO4 12 H2O 0.5 g

KNO3 0.5 g

MgSO4 7 H2O 0.4 g

NaCl 5.0 g

NaHCO3 (1M) 25.0 ml

Na2CO3 (1M) 5.0 ml

Distilled water 1000.0 ml

pH 9.09.5

Trace element solution (see below) 1 ml

Trace elements solution:

EDTA 5.0 g

CuCl2 2 H2O 0.1 g

FeSO4 7 H2O 2.0 g

ZnSO4 7 H2O 0.1 g

NiCl2 6 H2O 0.02 g

CoCl2 6 H2O 0.2 g

Na2MoO4 0.03 g

Distilled water 1000.0 ml

Prepare the medium without the NaHCO3 and Na2CO3. Basal medium, trace element, NaHCO3 (1M) and Na2CO3 (1M) sterilize separately at 121C 15 min. When preparing liquid media cool the mineral salts solution, NaHCO3 and Na2CO3 solutions to room temperature before mixing. When preparing agar add 2.0 % agar to the mineral salt solution and autoclave. Cool NaHCO3 and Na2CO3 stock solutions and agar to 50-55C before mixing.

 

Updated 12.07.2021