390. SULFURIMONAS MEDIUM
NH4Cl 0.33 g
KCl 0.33 g
CaCl2 × 2H2O 0.03 g
KH2PO4 0.16 g
MgCl2 × 6H2O 0.33 g
NaCl 10.0 g
NaHCO3 2.0 g
Elemental sulfur 5.0 g
KNO3 1.0 g
Trace element solution (see below) 1 mL
Vitamin solution (see below) 1 mL
pH of 7.5–8.0 (measured at 25 °C)
Distilled water 1000.0 ml
Trace element solution SL-10:
HCl (25%; 7.7 M) 10.0 ml
FeCl2 × 4 H2O 1.5 g
ZnCl2 70.0 mg
MnCl2 × 4 H2O 100.0 mg
H3BO3 6.0 mg
CoCl2 × 6 H2O 190.0 mg
CuCl2 × 2 H2O 2.0 mg
NiCl2 × 6 H2O 24.0 mg
Na2MoO4 x 2 H2O 36.0 mg
Distilled water 990.0 ml
Vitamin solution:
Biotin 2.0 mg
Folic acid 2.0 mg
Pyridoxine 0.1 mg
Riboflavin 5.0 mg
Pantotenoic acid 5.0 mg
p-Aminobenzoic acid 5.0 mg
Thiamine-HCl 5.0 mg
Nicotinic acid 5.0 mg
Vitamin B12 0.1 mg
Lipoic acid 5.0 mg
Distilled water 1000.0 ml
pH 7.2-7.4.
The anoxic medium is prepared by boiling and cooling it under N2 flow. No reducing agents are added. Elemental sulfur as electron donor is added to the Hungate tubes prior sterilization; potassium nitrate from anoxic sterile stock solution was added as electron acceptor just before inoculation.
Updated 15.07.2021