40. AZOTOBACTER MEDIUM 2

KH₂PO₄ 0.2 g

K₂HPO₄ 0.8 g

MgSO₄ × 7 H₂O 0.2 g

CaSO₄ × 2 H₂O 0.1 g

FeCl₃ Traces

Na₂MoO₄ Traces

Yeast extract 0.5 g

Sucrose 20.0 g

Agar 15.0-20.0 g

Distilled water 1000.0 ml

pH 7.2

Autoclave at 111°C for 30 min.