403. ALKALISPIRILLUM MOBILE MEDIUM (AMM)

NaCl 20.0 g

Na₂S₂O₃ 1.0 g

KH₂PO₄ 0.8 g

Na-acetate 2.0 g

Yeast extract 1.0 g

Trace elements (see below) 1.0 ml

MgCl₂ × 7 H₂O 0.1 g

CaCl₂ × 2 H₂O 0.05 g

NH₄Cl 0.8 g

Na₂CO₃ 21.2 g

NaHCO₃ (8 %) 168.0 ml

Distilled water 832.0 ml

Trace element solution :

FeCl₂ × 4 H₂O 1.8 g

CoCl₂ × 6 H₂O 250.0 mg

NiCl₂ × 6 H₂O 10.0 mg

CuCl₂ × 2 H₂O 10.0 mg

MnCl₂ × 4 H₂O 70.0 mg

ZnCl₂ 100.0 mg

H₃BO₃ 500.0 mg

Na₂MoO₄ × 2 H₂O 30.0 mg

Na₂SeO₃ × 5 H₂O 10.0 mg

Distilled water 1000.0 ml

For dissolving adjust pH to about 3 with 1 N HCl.

We recommend that liquid and solid media be made by autoclaving the Na₂CO₃ separately, otherwise the high pH will cause ammonia to be lost. When making media solidified with agar (20 g/l) the agar should also not be autoclaved with the Na₂CO₃ otherwise the agar will be hydrolysed. When mixing the ingredients for media cool all components to about 55 °C otherwise hydrolysis of the agar may occur. Adjust pH after autoclaving by adding the NaHCO₃ (8 %) to pH 9.0. Please note that the NaHCO₃ solution should be filter sterilized